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KMID : 0363320020230020181
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Journal of Korean Oriental Internal Medicine
2002 Volume.23 No. 2 p.181 ~ p.190
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Protective Effects of Omija-tang on H_2O_2-induced apoptotic death of H9c2 cardiomyoblast cells
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Choi Jin-Young
Han Myoung-Ah
Choi Woo-Jung
Kim Dong-Woung
Jung Dae-Young
Shin Sun-Ho
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Abstract
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The water extract of Omija-tang(OMJT) has been traditionally used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of OMJT protects cells from such damage. Therefore, this study was conducted to investigate the protective mechanisms of OMJT on H_2O_2-induced toxicity in H9c2 cardiomyoblast cells. Treatment of H_2O_2 markedly induced death of H9c2 cardiomyoblast cells in a dose-dependent manner. The characteristics of H_2O_2-induced death of H9c2 showed apparent apoptotic features, such as DNA fragmentation. However, OMJT significantly reduced both H_2O_2-induced cell death and chromatin fragmentation. The decrease of Bcl-XL expression by H_2O_2 was inhibited by OMJT. In addition, the increase of Bcl-XS and Bax expression were also inhibited by OMJT. In particular, Fas expression, which is generally recognized as cell death inducing signal by Fas/FasL interaction, was markedly increased by H_2O_2 in a time-dependent manner, whereas this increase was completely prevented by OMJT. The combined treatment of OMJT and H_2O_2 in H9c2 cells also reduced activation of caspase-9 and caspase-3 like protease.
Taken together, this study indicates that the protective effects of the water extract of OMJT against oxidative damage may be mediated by the modulation of BcI-XL/S and Bax expression by way of the regulation of mitochondrial membrane potential and caspase cascades.
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KEYWORD
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cardiomyopathy, Omija-tang
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